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Strain - JW4103-1Strain JW4103-1Strain JW4103-1
- CGSC#: 10954
- Strain Designation
- Sex(Hfr,F+,F-,or F'): F-
- 7 Mutations:
- Comments:
- From the Keio Collection of single-gene knockouts constructed through a collaboration of the Inst. of Adv. Biosci. at Keio Univ., the Nara Inst. of Sci. and Technol., Japan and Purdue Univ., USA
- There is some question as to if this strain has a complete knockout of groL
- While this strain is reported to have a duplicate functioning groL gene, the expression is greatly reduced (see Aich 2011 reference)
- References:
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
- 2011. Plasmid-mediated transformation of Escherichia coli having a dependence on the heat-shock protein GroEL Int. J. Genet. Mol. Biol. 3(3):51-54.