Strain - BW25113/pWTZ594

Strain - BW25113/pWTZ594Strain BW25113/pWTZ594

Strain BW25113/pWTZ594

Designation	Source	Preference
BW25113/pWTZ594		1

6 Mutations:

Mutation	Location	Certainty	Display
DE(araD-araB)567	1.40	1	Δ(araD-araB)567
lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
LAM-	17.40	1	λ^-
rph-1	82.20	1	rph-1
DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
hsdR514	98.74	1	hsdR514

Comments:
- This is the parent strain for the Keio Collection of single gene knockouts that contains the GFPuv expressing pWTZ594 plasmid
- This strain was originally reported to be lacI^Q, but it was later shown to be lacI⁺ (Baba 2006 ref. Supplementary Table 1)
Episome/Plasmid: pWTZ594
Plasmid Markers/Mutations:
bla(ApR)
lacZp-GFPuv(gfp)
ori[pMB1]
References:
- Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
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