Strain 20SO
- CGSC#: 5017
- Strain Designation
Designation Source Preference 20SO Paris Strain 1 EMG8 Clowes & Hayes 3 - Sex(Hfr,F+,F-,or F'): F-
- 10 Mutations:
Mutation Location Certainty Display lacZ43(Fs) 7.81 1 lacZ43(Fs) glnX44(AS) 14.99 1 glnX44(AS) LAM- 17.40 1 λ- mgl-50 48.17 1 mgl-50 galP63 66.53 1 galP63 rpsL135(strR) 74.84 1 rpsL135(strR) malT1(LamR) 76.54 1 malT1(λR) xyl-7 80.34 1 xyl-7 mtlA2 81.26 1 mtlA2 thiE1 90.34 1 thiE1 - Comments:
- EMG8 Clowes & Hayes Lab Manual.
- The strain gives a weak positive reaction on MacConkey-Mtl medium. The mtl-2 marker is not known to have reverted in other strains but it is possible that this strain is mtl+.
- This strain gives a strongly positive reaction on MacConkey-Ara medium. the ara-13 marker has reverted in many other strains and we conclude that it has reverted in this strain as well.
- References:
- Luria, S.E., J.N. Adams, R.C. Ting 1960. Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12:348-390
- Franklin, N.C., S.E. Luria 1961. Transduction by bacteriophage P1 and the properties of the lac genetic region in E. coli and S. dysenteriae. Virology 15:299-311
- Buttin, G. 1963. Mecanismes regulateurs dans la biosynthese des enzymes du metabolisme du galactose chez Escherichia coli K12. II. Le determinisme genetique de la regulation. J.Mol.Biol. 7:183-205
- Buttin, G. 1963. Mecanismes regulateurs dans la biosynthese des enzymes du metabolisme du glactose chez Escherichia coli K12. La biosynthese induite de la galactokinase et l'induction simultanee de la sequence enzymatique. J.Mol.Biol. 7:164-182
- Clowes, R., W. Hayes 1968. Experiments in microbial genetics
- Riordan, C., H.L. Kornberg 1977. Location of galP, a Gene Which Specifies Galactose Permease Activity, on the Escherichia coli Linkage Map Proc.Roy.Soc.Lond.B 198(1133):401-410.
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