Strain CR
- CGSC#: 5414
- Strain Designation
Designation Source Preference CR Denhardt D. 1 - Sex(Hfr,F+,F-,or F'): F-
- 6 Mutations:
Mutation Location Certainty Display glnV0(AS) 14.99 1 glnV0(AS) LAM- 17.40 1 λ- thyA0 63.86 1 thyA0 rpsL154(strR) 74.84 1 rpsL154(strR) ThyR-(deoB,deoC) 1000.00 1 ThyR-(deoB,deoC) phi Xs 1000.00 1 φXs - Comments:
- from a cross between Edgar's ``CR34 Hfr'' strain and Bertani's E. coli C strain C416.
- Not a pure K-12 strain; K-12 x E. coli C hybrid.
- We have not yet been able to trace the derivation of this strain completely. It can be used to transfer φX-sensitivity by transduction with xyl+, although it does not grow φX very well.
- References:
- Denhardt, DT, RL Sinsheimer 1965. The process of infection with bacteriophage phi-X174. IV. Replication of the viral DNA in a synchronized infection. J. Mol. Biol. 12(3):647-62.
- Denhardt, DT, DH Dressler, A Hathaway 1967. THE ABORTIVE REPLICATION OF PhiX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 57(3):813-820.
- McFadden, G, DT Denhardt 1974. Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein. J. Virol. 14(5):1070-5.
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