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Strain BGS376

• CGSC#: 8120
• Strain Designation

  Designation	Source	Preference
  BGS376	Murgola E.J.	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 5 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	1	λ-
  DE(tonB-trp)	28.00	1	Δ(tonB-trp)
  IN(rrnD-rrnE)1	73.74	1	IN(rrnD-rrnE)1
  rph-1	82.20	1	rph-1
  glyV55	94.63	1	glyV55

  
  
• Comments:
  • The episome is a derivative of the Fredericq F-prime and contains genes for colicins V and B and the tonB-cysB region of the chromosome that includes the trp operon, with a suppressible trpA mutation.
• Episome/Plasmid: F596-14

  Plasmid Markers/Mutations:
  colB+
  colI+
  colV+
  trpA236(Am,UAG234)

• References:
  • Arkov, A.L., A. Mankin, E.J. Murgola 1998. An rRNA fragment and its antisense can alter decoding of genetic information. J.Bacteriol. 180:2744-2748
  • Chernyaeva, N.S., E.J. Murgola, A.S. Mankin 1999. Suppression of nonsense mutations induced by expression of an RNA complementary to a conserved segment of 23S rRNA. J.Bacteriol. 181:5257-5262
  
  

  Printable Strain Report