CGSC Strain#: 5158
Strain Designation: D21     
Source of Strain: H.G. Boman
Sex: F-
Chromosomal Markers: proA23,
lac-28,
tsx-81,
trp-30,
his-51,
rpsL173(strR)
,
ampCp-1Strain Comments: - ampCp-1-- is a promoter mutation; AmpC+ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
- ampCp-1 was formerly called ampA1
- strR = streptomycin resist.
References: - Boman, HG, KG Eriksson-Grennberg, S Normark, E Matsson 1968. Resistance of Escherichia coli to penicillins. IV. Genetic study of mutants resistant to D,L-ampicillin concentrations o 100 mu-g-ml. Genet. Res. 12(2):169-85.
- Grundstrom, T., B. Jaurin, T. Edlund, S. Normark 1980. Physical mapping and expression of hybrid plasmids carrying chromosomal beta-lactamase genes of Escherichia coli K-12. J.Bacteriol. 143:1127-1134