CGSC Strain#: 4302
Strain Designation: KL717      Source of Strain: M.C. Cappello
Other Designations: KLF48/KL159
Sex: F' Episome/Plasmid: F148
   Plasmid Markers/Mutations: Δfli
Chromosomal Markers: Δ(gpt-proA)62, glnX44(AS), λ^-, aroD5, hisG4(Oc), gyrA12(NalR), recA1, xyl-7 or xylA5, thiE1, tsx-29 or tsx-1
Strain Comments:

• This strain must be maintained on medium containing Pro, Thi and lacking His, Trp, Tyr, Phe.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• Δ(gpt-proA)62 was formerly called proA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.
• Oc = ochre(UAA) mutation

References:

• Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.