CGSC Strain#: 6518
Strain Designation: NS2     
Source of Strain: M. Matsuhashi
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
secA206(aziR)
,
fhuA16,
lpcA2,
lacY1,
galT23,
λ-,
trp-84,
his-215,
thyA710,
rpsL263(strR)
,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- galT23-- : This is the standard gal6 of Morse & Lederberg.
- thyA710-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- aziR = azide resistance
- strR = streptomycin resist.
References: - Tamaki, S., T. Sato, M. Matsuhashi 1971. Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12. J.Bacteriol. 105:968-975