CGSC Strain#: 6552
Strain Designation: χ1849      Source of Strain: R. Curtiss
Other Designations: chi1849
Other Designations: χ1776-like
Other Designations: chi1776-like
Sex: F- Chromosomal Markers: fhuA53, dapD8, purE41, glnV42(AS), Δ(galK-uvrB)40, λ^-, minB-2, his-53, gyrA25(NalR), Δ(bioH-asd)29, metB65, cycA1, hsdR2, cycB2, ilv-277, oms-1, tte-1
Strain Comments:

• This strain is great grandparent of Χ1776; differs in being ilv-277, his-53, purE41 and Thy⁺, and lacking rfb-2 and osm-2
• dapD8-- Strains with this mutation require 100ug/ml diaminopimelic acid even when grown on LB
• minB-2-- mini-cell producer
• Δ(bioH-asd)29-- : inclusion of glpD in deletion also results in inability to grow on glycerol or glycerol-3-P as sole C source and stasis with gluconeogenic C-sources. See glpD mutants.
• Δ(bioH-asd)29-- : asd mutation causes requirement for DAP for growth on rich medium and DAP and Homoserine (or Threonine plus Methionine) on minimal. Deletion includes at least,BioH,yhgI,gntT,malQPT,rtcABR,glpRGED,glgPACXB,asd
• metB65-- is probably metB. Sometimes suggested to be metC.
• oms-1-- is an uncharacterized membrane defect.
• glnV42(AS) was formerly called supE42
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.

References:

• Hansen, JB, Y Abiko, R Curtiss 1981. Characterization of the Streptococcus mutans plasmid pva318 cloned into Escherichia coli. Infect. Immun. 31(3):1034-43.