CGSC Strain#: 6602
Strain Designation: FS131      Source of Strain: P.F. Sparling
Sex: Hfr    Point of Origin#: 12   Map Position: 68.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: rimG21, ksgA19, fhuA2::IS2, lacY1 or lacZ4, tsx-1, glnX44(AS), gal-6, λ^-, purF1, xyl-7, mtlA2, metB1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• purF1-- is a very stable mutation and may be an intragenic deletion.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rimG21 was formerly called ramB1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor

References:

• Sparling, P.F., Y. Ikeya, D. Elliot 1973. Two genetic loci for resistance to kasugamycin in Escherichia coli. J.Bacteriol. 113:704-710
• Zimmerman, R.A., Y. Ikeya, P.F. Sparling 1973. Alteration of ribosomal protein S4 by mutation linked to kasugamycin-resistance in Escherichia coli. Proc.Natl.Acad.Sci.USA 70:71-75