CGSC Strain#: 6728
Strain Designation: DF920      Source of Strain: D. Fraenkel
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, ompF627(T2R), pfkB20::Tn10, fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• pfkB20::Tn10-- The Tn 10 insertion was erroneously referred to as pkfB21::Tn 10 in Tabl -- e 1 of Daldal and Fraenkel 1981.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• = tetracycline resistant
• Const = constitutive
• T2R = T2 Resistant

References:

• Daldal, F., D. Fraenkel 1981. Tn10 insertions in the pfkB region of Escherichia coli. J.Bacteriol. 147:935-943
• Daldal, F., J. Babul, V. Guixe, D. Fraenkel 1982. An alteration in phosphofructokinase 2 of Escherichia coli which impairs gluconeogenic growth and improves growth on sugars. Eur.J.Biochem. 126:373-379