CGSC Strain#: 6769
Strain Designation: K165      Source of Strain: S. Cooper
Sex: F- Chromosomal Markers: lacZ53(Am), phoA5(Am), λ^-, tyrT47(OS), tyrT91(ts,AS), trp-48(Am), relA1, rpsL150(strR), malT66(Am,λ^R), rpoH601(Am), spoT1
Strain Comments:

• Other markers which might be in this strain - tonA22, ompF627(T2R), phoU35, bglCop16.
• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• phoA5(Am)-- is Garen's phoAH12.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rpoH601(Am) was formerly called htpR1
• tyrT91(ts,AS) was formerly called supC91
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• OS = ochre(UAA) suppressor
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Cooper, S, T Ruettinger 1975. A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12. Mol. Gen. Genet. 139(2):167-76.
• Neidhardt, F.C., R.A. VanBogelen 1981. Positive regulatory gene for temperature-controlled proteins in Escherichia coli. Biochem.Biophys.Res.Commun. 100:894-900