CGSC Strain#: 6771
Strain Designation: AKK221     
Source of Strain: I.R. Booth
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ-,
relA1,
spoT1,
ilv-299,
metB1,
zjc-2221::Tn10,
ampCp-1,
mcrB9999,
creC510Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- zjc-2221::Tn10-- cotransduces ~ 24% with melB and 0.4% with ampC.
- ampCp-1-- is a promoter mutation; AmpC+ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- ampCp-1 was formerly called ampA1
- creC510 was formerly called phoM510
- = tetracycline resistant
- Const = constitutive
References: