CGSC Strain#: 6862
Strain Designation: KH1366     
Source of Strain: T. Horiuchi
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: metD-88,
dnaQ49(ts)
,
Δ(cod-lacI)6,
tsx-7,
λ-,
srl-8,
relA1,
spoT1,
metB1Strain Comments: - (Ref. below to dnaQ.)
- dnaQ49(ts)-- Temperature sensitive on L without NaCl
- Δ(cod-lacI)6-- is Lederberg's lac-72 from strain W4032. It is lacI-Z-Y-. Feedback, 1999, indicates that it also deletes part or all of the cod operon.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- Δ(cod-lacI)6 was formerly called Δ6
- Δ(cod-lacI)6 was formerly called lac-3
- Δ(cod-lacI)6 was formerly called lac-72(del)
- dnaQ49(ts) was formerly called mutD49
- metD-88 was formerly called metD1
- ts = Temperature Sensitive
References: - Horiuchi, T., H. Maki, M. Sekiguchi 1978. A new conditional lethal mutator (dnaQ) in Escherichia coli K12. Mol.Gen.Genet. 163:277-283
- Horiuchi, T., H. Maki, M. Sekiguchi 1981. Conditional lethality of Escherichia coli strains carrying dnaE and dnaQ mutations. Mol.Gen.Genet. 181:24-28
- Takano, K., Y. Nakabeppu, H. Maki, T. Horiuchi, M. Sekiguchi 1986. Structure and function of dnaQ and mutD mutators of Escherichia coli. Mol.Gen.Genet. 205:9-13