CGSC Strain#: 7072
Strain Designation: A179     
Source of Strain: Russel & Model
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
trxB15::kan,
zca-1230::Tn10,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
trxA14::kan,
mcrB1,
creC510Strain Comments: - zca-1230::Tn10-- cotransduces 88% with aroA. PCR analysis (Nichols et al. 1998) places this insertion at nucleotide 946,252 (20.297 min.)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- trxA14::kan was formerly called fip-1
- trxA14::kan was formerly called fip::kan
- zca-1230::Tn10 was formerly called zbj-1230::Tn10
- = tetracycline resistant
- = kanamycin resistant
- Const = constitutive
- T2R = T2 Resistant
References: - Russel, M., P. Model 1985. Direct cloning of the trxB gene that encodes thioredoxin reductase. J.Bacteriol. 163:238-242
- Russel, M, P Model 1986. The role of thioredoxin in filamentous phage assembly. Construction, isolation, and characterization of mutant thioredoxins. J. Biol. Chem. 261(32):14997-5005.