CGSC Strain#: 4308
Strain Designation: KL218     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: lacY1 or lacZ4 or lacY29,
proC24,
tsx-63,
purE41,
λ-,
pyrF30,
his-53,
gyrA12(NalR)
,
thyA25,
argG34 or argG35,
rpsL97(strR)
,
xyl-7 or xyl-14,
mtlA2,
metB1,
metB65,
cycA1,
cycB2Strain Comments: - This strain contains no amber suppressors.
- thyA25-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- argG34 or argG35-- may be argG34 and argG35.
- metB65-- is probably metB. Sometimes suggested to be metC.
- NalR = Nalidix.acidResist.
- strR = streptomycin resist.
References: