CGSC Strain#: 4324
Strain Designation: JC5219     
Source of Strain: A.J. Clark
Other Designations: AB1157, Su-
Other Designations: ED85
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
λ-,
hisG4(Oc)
,
rpsL31(strR)
,
xylA5,
mtl-1,
rha-3,
argE3(Oc)
,
Met-(ts)
or Cys-0Strain Comments: - This strain was intended to be a suppressor-free derivative of AB1157. After a "supE+" revertant was selected, a requirement for CYS or MET appeared.
- We have received reports that the strain still carries a suppressor of some sort. The rha marker was introduced separately.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- Δ(gpt-proA)62 was formerly called proA2
- Am = amber(UAG) mutation
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: