CGSC Strain#: 4387
Strain Designation: KL283     
Source of Strain: M.C. Cappello
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
phoA4(Am)
,
serS14(ts)
,
ompF627(T2R)
,
serU132(AS)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
mcrB9999,
creC510,
serSo17Strain Comments: - phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- serU132(AS) was formerly called supD32
- serU132(AS) was formerly called Su1+
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Const = constitutive
- T2R = T2 Resistant
- ts = Temperature Sensitive
References: - Clarke, S.J., K.B. Low, W. Konigsberg 1973. Isolation and characterization of a regulatory mutant of an aminoacyltransfer ribonucleic acid synthetase in Escherichia coli K-12. J.Bacteriol. 113:1096-1103