CGSC Strain#: 4391
Strain Designation: KL219     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: lacY1 or lacZ4 or lacY29,
proC24,
tsx-63,
purE41,
λ-,
pyrF30,
his-53,
gyrA12(NalR)
,
recA1,
thyA25,
argG34 or argG35,
rpsL97(strR)
,
xyl-7 or xyl-14,
mtlA2,
metB1,
cycA1,
cycB2,
met-65Strain Comments: - This strain probably contains no amber suppressors. The lac marker is probably lacY29. The xyl marker is weak.
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- thyA25-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- argG34 or argG35-- may be argG34 and argG35.
- met-65-- is probably metB65, but it has also been suggested that it is metC.
- NalR = Nalidix.acidResist.
- strR = streptomycin resist.
References: