CGSC Strain#: 4434
Strain Designation: MC102     
Source of Strain: A. Markovitz
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
secA206(aziR)
,
fhuA23,
lacY1,
tsx-67,
lon-9,
galK2(Oc)
,
λ-,
trpE38,
rpsL109(strR)
,
xylA5,
mtl-1,
thiE1Strain Comments: - This strain has a tendency to die in the cold.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- lon-9-- = capR9 of Markovitz.
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- aziR = azide resistance
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Markovitz, A. 1964. Regulatory mechanism for synthesis of capsular polysaccharide in mucoid mutants of Escherichia coli K-12. Proc.Natl.Acad.Sci.USA 51:239-240