CGSC Strain#: 4498
Strain Designation: PL2      Source of Strain: G. Buttin
Other Designations: Q396
Other Designations: KA57
Other Designations: PL-2
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: galE28(GalS), λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• GalS = UDPGal accum.toxic if GalKRP+

References:

• Buttin, G. 1963. Mecanismes regulateurs dans la biosynthese des enzymes du metabolisme du glactose chez Escherichia coli K12. La biosynthese induite de la galactokinase et l'induction simultanee de la sequence enzymatique. J.Mol.Biol. 7:164-182
• Wu, HC, HM Kalckar 1966. Endogenous induction of the galactose operon in Escherichia coli K12. Proc. Natl. Acad. Sci. U.S.A. 55(3):622-9.