AB2462      Source of Strain:
F- Chromosomal Markers: thr-1
, thiE1Strain Comments:
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA12-- Missense Ser145(TCC) to Phe(TTC)
- recA12-- in AB2462, this is not a good allele; it is iffy Rec- and the strain is sicker than other recA- strains.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- glnX44(AS) was formerly called su+II
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- Howard-Flanders, P., L. Theriot 1966. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics 53:1137-1150
- Templin, A., L.J. Margossian, A.J. Clark 1978. Suppressiblitiy of recA, recB and recC mutations by nonsense suppressors. J.Bacteriol. 134:590-596