CGSC Strain#: 12115
Strain Designation: BW12556     
Source of Strain: B.L. Wanner
Sex: F-
Chromosomal Markers: phnJ175::Tn5-132,
Δ(argF-lac)169,
λ-,
rpoS396(Am)
,
rph-1,
hsdR514,
creC510,
[phnB]Strain Comments: - phnJ175::Tn5-132-- Tn5-132 insertion of the phn region from E. coli B
- Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
- Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- [phnB]-- functional phn locus from E. coli B
- creC510 was formerly called phoM510
- = tetracycline resistant
- Am = amber(UAG) mutation
- Const = constitutive
References: - Metcalf, W.W., B.L. Wanner 1991. Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi. J.Bacteriol. 173:587-600