CGSC Strain#: 12122
Strain Designation: RP437     
Source of Strain: J.S. Parkinson
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
fhuA31,
lacY1,
tsx-78,
λ-,
eda-50,
hisG4(Oc)
,
rfbC1,
rpsL136(strR)
,
xylA5,
mtl-1,
metF159(Am)
,
thiE1Strain Comments: - Advice from J.S.Parkinson: After reviving this strain from the filter, test on soft agar plates and pick a colony that is motile and chemotactic for your experiments, because nonmotile variants arise in room temperature stab cultures.
- This is the parent strain of many che and mot mutants. For questions about use or characteristics of this strain, contact parkinson@biology.utah.edu.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Parkinson, JS 1978. Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis. J. Bacteriol. 135(1):45-53.