CGSC Strain#: 6672
Strain Designation: JE5505      Source of Strain: Y. Hirota
Sex: F- Chromosomal Markers: Δ(gpt-proA)62, lacY1, tsx-29, glnX44(AS), galK2(Oc), λ^-, Δlpp-254, pps-6, hisG4(Oc), xylA5, mtl-1, argE3(Oc), thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Hirota, Y, H Suzuki, Y Nishimura, S Yasuda 1977. On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein-lipoprotein. Proc. Natl. Acad. Sci. U.S.A. 74(4):1417-20.