CGSC Strain#: 3059
Strain Designation: AB3059     
Source of Strain: P. Howard-Flanders
Sex: Hfr
Point of Origin#: 13
Map Position: 84.98
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: leuB6(Am)
,
lacZ4,
glnX44(AS)
,
λ-,
rfbC1,
thyA41,
rpsL8,
ilvD132,
thiE1,
deo-43Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- thyA41-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
References: - Howard-Flanders, P. 1968. Genes that control DNA repair and genetic recombination in Escherichia coli. Adv.Biol.Med.Physics 12:299-317