CGSC Strain#: 3271
Strain Designation: AB3271     
Source of Strain: A.J. Pittard
Sex: F-
Chromosomal Markers: Δ(gpt-proA)62,
lacY1,
tsx-358,
glnX44(AS)
,
aroG365,
galK2(Oc)
,
tyrR352,
aroH367,
hisG4(Oc)
,
rfbC1,
ilvC7,
argE3(Oc)
,
thiE1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- tyrR352-- : Note that the insertion of phage Mu at the tyrA locus, called tyrA1 by Taylor has also been called tyrA352::Mu (see strain AT2024).
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Wallace, B.J., J. Pittard 1969. Regulator gene controlling enzymes concerned in tyrosine biosynthesis in Escherichia coli. J.Bacteriol. 97:1234-1241