CGSC Strain#: 4266
Strain Designation: KL713     
Source of Strain: M.C. Cappello
Other Designations: KLF44
Sex: F'
Episome/Plasmid: F144
Plasmid Markers/Mutations: Chromosomal Markers: araC14,
leuB6(Am)
,
secA206(aziR)
,
fhuA23,
proC32,
tsx-67,
purE42,
glnX44(AS)
,
λ-,
trpE38,
recA1,
rpsL109(strR)
,
xylA5,
mtl-1,
metE70,
thiE1Strain Comments: - This strain must be maintained on medium containing thi, met, try and lacking ade, leu, pro.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal- phenotype, sup-78,near his.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- proC32 was formerly called proC65
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- aziR = azide resistance
- strR = streptomycin resist.
References: - Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.