CGSC Strain#: 4274
Strain Designation: JC411      Source of Strain: A.J. Clark
Sex: F- Chromosomal Markers: leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ^-, hisG1, rfbC1, galP63, argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Clark, A.J., A.D. Margulies 1965. Isolation and characterization of recombination-deficient mutants of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 53:451-459
• Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167
• Clark, A.J., W.K. Maas, B. Low 1969. Production of a merodiploid strain from a double male strain of E. coli K12. Mol.Gen.Genet. 105:1-15
• Shaw, CR, JN Baptist, DA Wright, TS Matney 1973. Isolation of induced mutations in E. coli affecting the electrophoretic mobility of enzymes. Mutat. Res. 18(3):247-50.
• Heard, JT, MA Butler, JN Baptist, TS Matney 1975. Chromosomal location of mutations affecting the electrophoretic mobility of malate dehydrogenase in Escherichia coli K-12. J. Bacteriol. 122(1):329-31.