CGSC Strain#: 4287
Strain Designation: KL718      Source of Strain: M.C. Cappello
Other Designations: F152/KL253
Sex: F' Episome/Plasmid: F152
   Plasmid Markers/Mutations: gal
Chromosomal Markers: galK35, λ^-, pyrD34, e14-, trpC45, his-68, tyrA2, recA1, rpsL118(strR), malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• This strain should be maintained on medium containing thi, ura, trp, his, tyr and with galactose as carbon source.
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• e14--- : Loss of the e14 element makes the strain McrA^-.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• LamR = Lambda-resistant
• strR = streptomycin resist.

References:

• Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.
• Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.