CGSC Strain#: 4501
Strain Designation: CS101-4U1     
Source of Strain: J.R. Beckwith
Other Designations: AT2243
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pyrE41,
spoT1,
metB1,
rrnB-2,
mcrB1,
creC510Strain Comments: - Called 3OsO U7 in reference below.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Beckwith, J.R., A.B. Pardee, R. Austrian, F. Jacob 1962. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J.Mol.Biol. 5:618-634