CGSC Strain#: 4527
Strain Designation: AT703     
Source of Strain: A.L. Taylor
Sex: F-
Chromosomal Markers: lacY1,
glnX44(AS)
,
gal-6,
manZ14,
hisG1,
purF59,
thyA3,
rpsL8 or rpsL104 or rpsL17,
malT1(λ
R)
,
pyrE41Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- thyA3-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- manZ14 was formerly called ptsM14
- manZ14 was formerly called mpt-4
- manZ14 was formerly called man-1
- = strep-resistant
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: - Taylor, A.L., C.D. Trotter 1967. Revised linkage map of Escherichia coli. Bacteriol.Rev. 31:332-353