CGSC Strain#: 4865
Strain Designation: H585     
Source of Strain: J. Adler
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
fhuA31,
lacY1,
tsx-78,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
fliC585,
hisG4(Oc)
,
rfbC1,
mgl-51,
rpsL136(strR)
,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- fliC585 was formerly called hag-585
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Armstrong, J.B., J. Adler 1969. Loction of genes for motility and chemotaxis on the Escherichia coli genetic map. J.Bacteriol. 97:156-161