CGSC Strain#: 4928
Strain Designation: P678-54     
Source of Strain: H.I. Adler
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
secA208,
fhuA2::IS2,
lacY1,
glnX44(AS)
,
gal-6,
λ-,
minB-2,
hns-1,
rfbC1,
galP63,
rpsL135(strR)
,
fic-1,
malT1(λ
R)
,
xyl-7,
mtlA2,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- secA208-- : azi is now secA
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- minB-2-- mini-cell producer
- fic-1-- Found in W677 and its derivatives. Mutant phenotype is filamentation in the presence of cAMP.
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- hns-1 was formerly called osmZ1
- hns-1 was formerly called cur-1
- rfbC1 was formerly called rfbD1
- secA208 was formerly called azi-8
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: - Adler, H.I., W.D. Fischer, A. Cohen, A.A. Hardigree 1967. Miniature Escherichia coli cells deficient in DNA. Proc.Natl.Acad.Sci.USA 57:321-326
- Inselburg, J 1970. Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli. J. Bacteriol. 102(3):642-7.
- Frazer 1975. Curr.Topics Microbiol.Immunol. 69:1-0