CGSC Strain#: 5173
Strain Designation: TP1      Source of Strain: H.G. Boman
Sex: F- Chromosomal Markers: proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), ampCp-1, ampC11
Strain Comments:

• This strain does not grow on minimal medium plus His, Trp and Pro.
• ampCp-1-- is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• ampC11-- has been called ampC1. It makes this strain beta-lactamase negative (and sensitive to Amp at concentrations greater than 1 ug/ml.
• ampCp-1 was formerly called ampA1
• strR = streptomycin resist.

References:

• Burman, L.G., J.T. Park, E. Lindstrom, H.G. Boman 1973. Resistance of Escherichia coli to penicillins: identification of the structural gene for the chromosomal penicillinase. J.Bacteriol. 116:123-130
• Jaurin, B., T. Grundstrom, T. Edlund, S. Normark 1981. The E. coli beta-lactamase attenuator mediates growth rate-dependent regulation. Nature 290:221-225