CGSC Strain#: 5361
Strain Designation: NH4939     
Source of Strain: P. Howard-Flanders
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
lacZ36,
proC32,
λ-,
sup-78(Mal+)
,
hisF860(Stable)
,
recA44(ts)
,
thyA54,
rpsE2115(SpcR)
,
rpsL109(strR)
,
malA38,
xylA5,
mtl-1,
metE70,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- sup-78(Mal+)-- is an uncharacterized mutation near his which confers a Mal+ phenotype.
- recA44(ts)-- is a temperature-sensitive mutation.
- recA44(ts)-- G to A at Nuc. 977 (Val 247 Met)
- thyA54-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- malA38-- : In Χ65 a Mal+ phenotype may have resulted from suppression of malA1, but in the absence of evidence, we show the mutations as malA38 and sup-78.
- proC32 was formerly called proC65
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- SpcR = spectinomycin resistant
- Stable = stable, non-reverting
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Hall, JD, P Howard-Flanders 1975. Temperature-sensitive recA mutant of Escherichia coli K-12: deoxyribonucleic acid metabolism after ultraviolet irradiation. J. Bacteriol. 121(3):892-900.