CGSC Strain#: 5760
Strain Designation: JE5550      Source of Strain: Y. Hirota
Other Designations: F506/JE5519
Sex: F' Episome/Plasmid: F506
   Plasmid Markers/Mutations: manA , pps
Chromosomal Markers: lacY1, tsx-29, glnX44(AS), galK2(Oc), manA4, aroD6, gyrA12(NalR), recA1, rpsL700(strR), mtl-1, argE3(Oc)
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Hirota, Y, H Suzuki, Y Nishimura, S Yasuda 1977. On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein-lipoprotein. Proc. Natl. Acad. Sci. U.S.A. 74(4):1417-20.
• Movva, NR, E Katz, PL Asdourian, Y Hirota, M Inouye 1978. Gene dosage effects of the structural gene for a lipoprotein of the Escherichia coli outer membrane. J. Bacteriol. 133(1):81-4.