CGSC Strain#: 6455
Strain Designation: YYC100     
Source of Strain: J.E. Cronan
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
secA214(aziR)
or secA216,
Δ(aroP-aceF)73,
zac-284::Tn10,
ts100,
lacZ36,
proC32,
tsx-67,
purE42,
glnX44(AS)
,
λ-,
trpE38,
lysA23,
xylA5,
mtl-1,
metE70,
thiE1,
fhuA23 or fhuA54Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- zac-284::Tn10-- cotransduces ca. 99% with aceEF; in YYC100, see comment about ts mutation, not present in YYC186.
- ts100-- : YYC100 has been reported to be temperature sensitive at 42C with the temperature sensitivity co-transduced with zac-284::Tn10. The zac-284::Tn10 strain YYC186 is not TS.
- purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal- phenotype, sup-78,near his.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- proC32 was formerly called proC65
- thiE1 was formerly called thi-1
- = tetracycline resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
References: