CGSC Strain#: 12380
Strain Designation: FS1461      Source of Strain: F. Stahl
Other Designations: C600/pMB4
Sex: F- Episome/Plasmid: pMB4
   Plasmid Markers/Mutations: bla(ApR)
   Plasmid Comment: This plasmid is derived from pMB1 and contains the EcoRI restriction/modification system.
Chromosomal Markers: thr-1, leuB6(Am), fhuA21, cyn-101, lacY1, glnX44(AS), λ^-, rfbC1, glpR200(glp^c), thiE1
Strain Comments:

• C600 and some of its derivatives have been reported to be e14- (and therefore mcrA^-). It appears that some subcultures of C600 lost the e14 element. It has not yet been determined if this strain carries e14
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• cyn-101 was formerly called cyn-1
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• glp^c = constitutive expr.glp regulon
• ApR = ampicillin resistant

References:

• Appleyard, R.K. 1954. Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K12. Genetics 39:440-452