S17-1 λpir/pLFT      Source of Strain:
T. RomeoOther Designations:
S17-1 Lambda pir/pLFTOther Designations:
pLFT Plasmid Markers/Mutations: Chromosomal Markers: RP4-2(Km::Tn7,Tc::Mu-1)
, creC510Strain Comments:
- RP4-2(Km::Tn7,Tc::Mu-1)-- integrated plasmid is Kan and Tet sensitive, due to the insertions in Tc and Km, and the Tn7 results in TRIM/aminopterin resistance and low level strR.
- RP4-2(Km::Tn7,Tc::Mu-1)-- The RP4-2 also has an ISR1 insertion element within the Tn135 transposon that inactivates the adjacent ampicillin resistance gene.
- pro-82-- is in proA or proB.
- LAMpir-- Allows the replication of plasmids with an R6Kγ origin of replication
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- endA1-- G to A transition mutation resulting in E208K amino acid substitution.
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- thiE1 was formerly called thi-1
- = good miniprep dsDNA quality
- Const = constitutive
- Edwards, AN, LM Patterson-Fortin, CA Vakulskas, JW Mercante, K Potrykus, D Vinella, MI Camacho, JA Fields, SA Thompson, D Georgellis, M Cashel, P Babitzke, T Romeo 2011. Circuitry linking the Csr and stringent response global regulatory systems. Mol. Microbiol. 80(6):1561-80.