CGSC Strain#: 6494
Strain Designation: DP50, supF     
Source of Strain: D. Pereira
Other Designations: χ2098
Other Designations: chi2098
Sex: F-
Chromosomal Markers: fhuA53,
dapD8,
lacY1 or Δ(cod-lacI)6,
glnX44(AS)
,
Δ(gal-uvrB)47,
λ-,
tyrT58(AS)
,
gyrA29(NalR)
,
ΔthyA57,
hsdS3Strain Comments: - dapD8-- Strains with this mutation require 100ug/ml diaminopimelic acid even when grown on LB
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- ΔthyA57-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- tyrT58(AS) was formerly called supF58
- AS = amber(UAG) suppressor
- NalR = Nalidix.acidResist.
References: - Leder, P, D Tiemeier, L Enquist 1977. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system. Science 196(4286):175-7.