CGSC Strain#: 6499
Strain Designation: DM1415      Source of Strain: D.W. Mount
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, tsx-33, glnX44(AS), galK2(Oc), λ^-, sulA211, recA1, rpsL31(strR), xylA5, mtl-1, thiE1, lexA3(Ind-), lexA51
Strain Comments:

• The mutations include a TS requirement for isoleucine and valine.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• sulA211-- was formerly called sfiA11.
• sulA211-- This allele is the result of a T>C change in the -10 promoter (TACTGT -> TACTGC), which also overlaps with the LexA-binding site (TACTGTACATCCATACAGTA -> TACTGCACATCCATACAGTA)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• lexA3(Ind-)-- : Non-cleavable LexA repressor protein; defective in SOS induction, UV sensitive
• lexA3(Ind-)-- This mutation results in a G85D amino acid substitution at the lexA cleavage site.
• lexA51-- : defective LexA resulting in constitutive expression of LexA-repressed genes. Formerly called spr-51
• lexA51-- This is a frameshift mutation from an single A deletion between bases 475 and 480
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Ind- = non-inducible
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Mount, DW 1977. A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways. Proc. Natl. Acad. Sci. U.S.A. 74(1):300-4.