CGSC Strain#: 6172
Strain Designation: NK5648     
Source of Strain: N. Kleckner
Sex: F'
Episome/Plasmid: F128-12
Plasmid Markers/Mutations: lacIp-4000(lacIQ),
lacZp-4008(L8),
lac-::Tn9 Plasmid Comment: Carries Tn9 inserted into lacZ or lacY. Derivation not known to us; episome was described as F'laci^QlacL8pro with Tn9 in lacY,Z
Chromosomal Markers: araBAD-0,
Δ(gpt-lac)5,
glnX44(AS)
,
metB-,
argE-(Am)
,
rpoB0(rifR)
,
thiE1Strain Comments: - The ara and rpo alleles are not identified because of the unknown derivation of the strain.
- The episome carries Tn 9 inserted into lacZ or lacY. We do not know the derivation of this strain. The F' was described to us as follows: lacIQL8 pro with Tn9 in lacYZ
- Δ(gpt-lac)5-- is the deletion Δ( pro-lac)X111 of Jacob and originated in the strain Hfr 3000 X111.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- argE-(Am)-- is suppressible by all of the characterized amber and ochre suppressors in Miller et a. 1977
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- rifR = rifamycin resistance
- = chloramphenicol resistant
References: