CGSC Strain#: 6614
Strain Designation: BJW72      Source of Strain: B. Smith-White
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ^-, Rac-0, xthA3, zdj-276::Tn10, hisG4(Oc), rfbC1, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• zdj-276::Tn10-- cotransduces 7% with pps, 32% with pheS and 67% with xthA. Placed by PCR (Nichols et al. 1998) in orf b1754 (ynjB), at nucleotide 1,834,488 (39.543 min.)
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = tetracycline resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

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