CGSC Strain#: 703
Strain Designation: W3646      Source of Strain: A. Richter
Sex: F- Chromosomal Markers: lacY1, glnX44(AS), galK2(Oc), rpsL38(strR), metB1, malB5::Sfa(att)
Strain Comments:

• When made F+ this strain gives rise to an Hfr with a point in the malB locus with high frequency.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• malB5::Sfa(att)-- sfa-3 is a sex factor-attachment-site in malB. When infected with F, this strain gives rise to an Hfr with the point of origin of Richter's Hfr3 (in malB).
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

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