CGSC Strain#: 12854
Strain Designation: SX1299      Source of Strain: S. Xie
Sex: F- Chromosomal Markers: Δ(argF-lac)169, gal-490, Δ(modF-ybhJ)803, λ[cI857 Δ(cro-bioA)], barA791-YFP(::cat), IN(rrnD-rrnE)1, rph-1
Strain Comments:

• CGSC Plate 5 Well D7
• This strain requires a Material Transfer Agreement from Harvard University prior to receiving the strain from the CGSC. Please contact Harvard University Office of Technology Development.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• gal-490-- This mutation has an IS2 insertion element in the mRNA leader of the gal operon preventing transcription.
• Δ(modF-ybhJ)803-- the deletion is about 13.7Kb and goes from the inergenic region between galE and modF to the intergenic region between ybhJ and ybhC.
• Δ(modF-ybhJ)803-- The sequence flanking the deletion is CGTAAACGCCTTATCCGGCCTACGGTTCGA Δ CGCATGCAGGCATGAAACCGCGTCTTTTTTC
• λ[cI857 Δ(cro-bioA)]-- In this prophage, the pL operon is intact and controlled by the temperature-sensitive Lambda repressor (cI857). A deletion of the right arm of the prophage from cro through the right attachment site, and extends into the bacterial bioA gene
• barA791-YFP(::cat)-- This allele is a protein fusion to the Venus YFP followed by the chloramphenicol resistance gene cat under its own promoter.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Δ(modF-ybhJ)803 was formerly called

References:

• Taniguchi, Y, PJ Choi, GW Li, H Chen, M Babu, J Hearn, A Emili, XS Xie 2010. Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329(5991):533-8.