CGSC Strain#: 1918
Strain Designation: AB1918      Source of Strain: G. Eggertsson
Other Designations: DE22
Sex: Hfr    Point of Origin#: 13   Map Position: 84.98   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: thr-1, leuB6(Am), lacZ4, glnX44(AS), λ^-, Δhis-22, purF1, rpsL8, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• Δhis-22-- is a deletion of the entire his operon.
• purF1-- is a very stable mutation and may be an intragenic deletion.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Garrick-Silversmith, L., P.E. Hartman 1970. Histidine-requiring mutants of Escherichia coli K12. Genetics 66:231-244