CGSC Strain#: 2544
Strain Designation: AB2544      Source of Strain: G. Eggertsson
Sex: F- Chromosomal Markers: lacY1, tsx-59, glnX44(AS), gal-6, LAMdgallysT102, hisG1, thyA3, argG6, xyl-7, ilvD188(Oc)
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• LAMdgallysT102-- : The lysT102 mutation was formerly called supL2.
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• thyA3-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• xyl-7 was formerly called xylA7
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Eggertsson, G., E.A. Adelberg 1965. Map positions and specificities of suppressor mutations in Escherichia coli K-12. Genetics 52:319-340