CGSC Strain#: 13594
Strain Designation: BKT20      Source of Strain:
Sex: F- Chromosomal Markers: λ^-, ΔymdB787::kan, ΔclsC888, IN(rrnD-rrnE)1, rph-1
Strain Comments:

• ΔymdB787::kan-- The primers used to make this knockout were AAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGATTCCGGGGATCCGTCGACC and GCAGCACCGCGCTCGCCAGCCGGGGCAAATCATTCATCTCCTTGTTGGGTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

References:

• Tan, BK, M Bogdanov, J Zhao, W Dowhan, CR Raetz, Z Guan 2012. Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates. Proc. Natl. Acad. Sci. U.S.A. 109(41):16504-9.