CGSC Strain#: 13596
Strain Designation: BKT29     
Source of Strain: Sex: F-
Chromosomal Markers: ΔaraBAD-714,
rcsF1386::mini-Tn10cam,
λ-,
ΔclsB861,
ΔymdB787::kan,
ΔclsC888,
ΔclsA856,
lpp-2,
Δ pgsA792,
rph-1Strain Comments: - ΔaraBAD-714-- : Deletes most of the araBAD operon.
- ΔymdB787::kan-- The primers used to make this knockout were AAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGATTCCGGGGATCCGTCGACC and GCAGCACCGCGCTCGCCAGCCGGGGCAAATCATTCATCTCCTTGTTGGGTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = kanamycin resistant
- = chloramphenicol resistant
References: - Tan, BK, M Bogdanov, J Zhao, W Dowhan, CR Raetz, Z Guan 2012. Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates. Proc. Natl. Acad. Sci. U.S.A. 109(41):16504-9.